Gram staining procedure, artwork
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Gram staining procedure, artwork
Gram staining procedure, artwork. Gram staining is used to differentiate between two groups of bacteria. Gram-positive bacteria are stained dark blue by the process, whereas Gram negative are unaffected. From top left to bottom right: 1) A sample of bacteria is smeared onto a glass slide. 2) The smear is dried slowly and heat-fixed to kill the bacteria. 3) The slide is flooded with gentian violet and left for 1 minute. 4) The gentian violet is washed off. 5) An iodine solution is added to aid penetration and retention of the stain. 6) After 30 seconds the slide is rinsed with ethanol, which washes the iodine out of some of the bacteria. 7) A red counter-stain (fuschin or eosin) is added to stain the Gram-negative bacteria. 8) The counter-stain is left for 30-60 seconds and then rinsed. 9) A drop of immersion oil is added to the slide, which is now ready to place under a microscope
Science Photo Library features Science and Medical images including photos and illustrations
Media ID 6324057
© PETER GARDINER/SCIENCE PHOTO LIBRARY
Apparatus Bacteria Bacterial Bacteriology Bacterium Diagrams Illustrated Method Micro Organism Micro Organisms Procedure Process Step Steps Technique Bio Chemistry Biochemical Empirical Laboratory Micro Biology Microbiological
EDITORS COMMENTS
This artwork captures the step-by-step process of the Gram staining procedure, a fundamental technique used in bacteriology and microbiology. Against a clean white background, the illustration showcases nine distinct stages involved in differentiating between two groups of bacteria. Starting from the top left corner and progressing to the bottom right, each step is meticulously depicted. The first stage shows a sample of bacteria being smeared onto a glass slide, followed by slow drying and heat-fixing to effectively kill off any living organisms. Next, gentian violet is flooded onto the slide for one minute before being washed off. To aid penetration and retention of the stain, an iodine solution is added at stage five. After 30 seconds, ethanol rinses away some of the iodine from certain bacteria while leaving others unaffected. At this point, a red counter-stain (fuschin or eosin) is introduced to specifically target Gram-negative bacteria. The counter-stain remains on the slide for 30-60 seconds before being rinsed off. Finally, a drop of immersion oil is carefully added to prepare the slide for microscopic examination. With its precise detailing and vibrant colors against a pure white backdrop, this artwork beautifully encapsulates an essential laboratory procedure that has revolutionized our understanding of bacterial classification and identification throughout history.
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